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2 edition of Monoseptic immobilisation of Bacillus subtilis for the production of alpha-amylase found in the catalog.

Monoseptic immobilisation of Bacillus subtilis for the production of alpha-amylase

George Argirakos

Monoseptic immobilisation of Bacillus subtilis for the production of alpha-amylase

by George Argirakos

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Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (M.Phil)-University of Birmingham, Dept of Chemical Engineering, 1989.

Statementby George Argirakos.
ID Numbers
Open LibraryOL13891258M

Abstract. The genus Bacillus comprises a heterogeneous group of chemoorganotrophic, aerobic, rod-shaped microorganisms. These include both mesophilic and thermophilic species as well as acidophiles and alkalophiles. One of their main characteristics is their ability to produce heat-resistant endospores. Growth activity and alpha-amylase production: Bacillus subtilis, Bacillus licheniformis and Lactobacillus acidophilus to study their effect on maize starch hydrolysis (6 used & new offers) Biosorption of heavy metals by free and immobilized cells of Pseudomonas aeruginosa and Bacillus subtilis isolated from garden soils. by Prem.

Kallio P, Palva A, Palva I. Enhancement of α-Amylase production by integrating and amplifying the α-Amylase gene of Bacillus amyloliquefaciens in the genome of Bacillus subtilis. Appl Microbiol Biotechnol ; 27(1): 1 Study of the optimum growth conditions of Bacillus subtilis (strain WT ), and Pseudomonas fluorescens (strain SBW25) By iGEM Toulouse Objective: determine what are the best temperature (between 30°C and 37°C) and pH (between 4 and 8) for the growth of these strains. 1. Determination of the combined influence of the pH and the temperature on the.

Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants and humans. A member of the genus Bacillus, B. subtilis is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. B. subtilis has historically been classified as. Two-step Bacillus subtilis Transformation Procedure - Based on “Molecular Biological Methods for Bacillus”- Preparation of Bacillus subtilis competent cell 1. Streak out the strain to be made competent on an LB or TBABG [Trytose blood agar base (Difco) + % glucose] agar plate as a large patch and incubate overnight at 30℃ 2.


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Monoseptic immobilisation of Bacillus subtilis for the production of alpha-amylase by George Argirakos Download PDF EPUB FB2

Argirakos, G., Thayanithy, K., & Wase, D. Effect of immobilization on the production of alpha- amylase by an industrial strain of Bacillus-Amyloliquefaciens. Journal of Chemical Technology and Biotechnology, 53(1), 33– PubMed Google ScholarCited by: 1.

The effect of immobilisation of an industrial strain of Bacillus amyloliquefaciens in calcium alginate beads on production of alpha-amylase was investigated using lactose-based media in shake.

Organized into 11 chapters, the book begins with the genetic map of Bacillus subtilis, followed by DNA replication and RNA polymerase of the said species. The book then describes the translational apparatus of Bacillus subtilis.

Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. Mar; 33 (1)– [PMC free article] Schmitt R, Freese E. Curing of a sporulation mutant and antibiotic activity of Bacillus subtilis. J Bacteriol. Oct; 96 (4)– [PMC free article] Walker JE, Abraham by: 3.

The activity of the free enzyme ended in 30 days, whereas the activity of the immobilized enzyme lasted for 60 days at storage conditions. alpha-Amylase immobilized on matrix maintained 40% of its.

Bacillus subtilis biofilm induction by plant polysaccharides Pascale B. Beauregarda, Yunrong Chaib,1, Hera Vlamakisa, Richard Losickb, and Roberto Koltera,2 aDepartment of Microbiology and Immunobiology, Harvard Medical School, Boston, MA ; and bDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA Edited by Sharon R.

Long, Stanford University. Thus, the aim of this work was to test the viability of production of protease, alkaline lipase and α-amylase by a strain of Bacillus subtilis LB5a using cassava wastewater as substrate instead of a synthetic medium with inductor. MATERIALS & METHODS.

3 Abstract Microbes play a key role in the pathology of inflammatory bowel disease even though the cause remains unknown. Here we report that Caenorhabditis elegans live longer when first exposed to the Bacillus subtilis compared to animals exposed to pathogenic fungus Candida albicans alone, suggesting a probiotic effect of B.

subtilis biofilm. This result was bolstered by the finding that. bacteria, Bacillus sp is widely used for thermostable -amylase production to meet industrial needs. They are known to be good producers of thermo stable -amylase, and these have been widely used for commercial production of the enzyme for various applications.

Immobilized enzymes are becoming increasingly popular as reusable, Selective. maximum production of antibiotics by immobilized whole cells of B. subtilis. Immobilized whole cells of B. subtilis were incubated at 30 oC in an orbital shaker at rpm and sample was drawn at 0 hour and then after every 4 hours, from 4 hours to 20 hours in case of time optimization, and at 0 hour and.

Supersmart - Bacillus Subtilis Billion CFU - Probiotic Strain - Improve Natural Defences & Help with External Infection | Non-GMO - 60 DR Capsules out of 5 stars 24 $ $ 00 ($/Count).

Bacillus subtilis is also known as Hay bacillus or Grass bacillus. Bacillus subtilis is gram positive, rod shape, motile, spore forming bacteria. Bacillus subtilis commonly present in soil which is human pathogens. The work describes the production of alkaline protease in a lab scale fermentor from Bacillus subtilis IH VaseeKaran et al.

() reported that the highest ratios of starch degradation ranged from to 4 for tested isolates, while Alkando and Ibrahim () indicated that the ratio of starch degradation by B. licheniformis was compared to the other tested bacterial species. Thermophilic microorganisms were capable of producing thermostable enzyme reported by Rasooli et al.

Batch and repeat batch fermentation were performed to evaluate the potential of date syrup and immobilized cells technique for the economical production of α-amylase. The enzyme production by immobilized cells was greater than that of freely suspended cells. Khlas date syrup gave the highest enzyme yield ( Uml-1) using the immobilized technique after 48 h of fermentation.

subtilis. and A. niger. after which an assay was carried out based on Dinitrosalicyclic acid method (DNSA), (Bertrand. et al., ). Effect of pH on the Production of Amylase. The effect of pH on the amylase production was carried out using the following pH values of 6,7,8,9, and 10 for.

subtilis. pH values and pH values 4,5,6,7and 8. El-Banna TE, Abdel-Aziz AA, Abou-Dobara MI, Ibrahim RI. Production and immobilization of α-amylase from Bacillus subtilis.

Pak J Biol Sci ;– Abdel-Naby MA, Hashem AM, Esawy MA, Abdel-Fattah AF. Immobilization of Bacillus subtilis α-amylase and characterization of its enzymatic properties.

Microbiol Res ;– B. subtilis, like other closely related species in the genus, B. licheniformis, B. pumulis, and B.

megaterium, have been shown to be capable of producing lecithinase, an enzyme which disrupts membranes of mammalian cells.

However, there has not been any correlation between lecithinase production and human disease in B. subtilis. Bacillus subtilis: Soil Organism or Probiotic?Or Both. Bacillus subtilis means thin and rod-shaped and it was first discovered in the ’s.

It is one of the best-characterized species of all the Gram-positive bacteria. Like other Bacillus species, it forms endospores when conditions are not favorable. It is able to grow well in oxygenated environments but can adapt to low-to-no oxygen.

Bacillus subtilis has become widely adopted as a model organism for laboratory studies and is one of the best understood prokaryotes in terms of molecular and cellular biology. Its superb genetic amenability and relatively large size have provided powerful tools to investigate a bacterium in all possible aspects.

This valuable reference work provides a comprehensive and up-to-date analysis of. Bacillus subtilis, Bacillus licheniformis, and Bacillus natto are commonly used probiotic preparations.

Dietary supplementation of single-strain probiotics can significantly improve animal production performance, regulate intestinal microflora imbalance, and enhance immune functions (11).

However, dietary supplementation. The use of Bacillus subtilis in synthetic biology and metabolic engineering is highly desirable to take advantage of the unique metabolic pathways present in this organism.

To do this, an evaluation of B. subtilis’ intrinsic biological parts is required to determine the best strategies to accurately regulate metabolic circuits and expression of target proteins.Bacillus subtilis is a ubiquitous naturally occurring saprophytic bacterium that is commonly recovered from soil, water, air and decomposing plant material.

Under most conditions, however, it is not biologically active and is present in the spore form. Different strains of B. sub-tilis can be used as biological control. A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp.

and identified as Bacillus subtilis BI The effect of various fermentation conditions on amylase production through shake-flask culture was .